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Uptake and metabolism of radioactive gibberellins by barley aleurone layers

โœ Scribed by Alan Musgrave; Sandra E. Kays; Hans Kende

Publisher
Springer-Verlag
Year
1971
Tongue
English
Weight
490 KB
Volume
102
Category
Article
ISSN
0032-0935

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โœฆ Synopsis

When aleurone layers were treated with labeled gibberellin A1 ((3)H-GA1), gibberellin A5 ((3)H-GA5) and the methyl ester of (3)H-GA5 ((3)H-GA5-ME), radioactivity was accumulated by the tissue for a period of 20-30 h. After this time, radioactivity was released into the medium. Concomitantly, ribonuclease was also liberated by the tissue. The radioactivity accumulated by aleurone layers was associated with polar metabolites of the respective GAs, and the extent of extent of accumulation was a function of the degree of GA metabolism (GA5-ME>GA5>GA1). Accumulation of radioactivity was inhibited in the cold and by the metabolic poisons NaF and dinitrophenol. This was thought to be due to inbition of GA metabolism. The accumulation of (3)H-GA1 in aleurone tissue did not reach saturation when unlabeled GA3 up to 10(-2) M was added to the incubation medium.

๐Ÿ“œ SIMILAR VOLUMES

Uptake and metabolism of3H-Gibberellin A
Uptake and metabolism of3H-Gibberellin A1by barley aleurone layers: Response to abscisic acid
โœ Ronn Nadeau; Lawrence Rappaport; Charles F. Stolp ๐Ÿ“‚ Article ๐Ÿ“… 1972 ๐Ÿ› Springer-Verlag ๐ŸŒ English โš– 524 KB

When barley aleurone layers were incubated with (3)H-Gibberellin A1 ((3)H-GA1), the hormone was converted to (3)H-GA-X (not identified), (3)H-GA8 and two other compounds tentatively identified as (3)H-GA1-glucoside, and (3)H-GA8-glucoside. Uptake and metabolism of the (3)H-GA1 were markedly enhanced