Uptake and incorporation of myo-inositol by bovine preimplantation embryos from two-cell to early blastocyst stages
✍ Scribed by A.C. Hynes; J.M. Sreenan; M.T. Kane
- Publisher
- John Wiley and Sons
- Year
- 2000
- Tongue
- English
- Weight
- 104 KB
- Volume
- 55
- Category
- Article
- ISSN
- 1040-452X
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✦ Synopsis
The uptake of myo-inositol and its incorporation into the phosphoinositides and inositol phosphates of the phosphatidylinositol (PtdIns) signal transduction system by in vivo preimplantation cattle embryos was investigated using [ 3 H] myo-inositol. Uptake of inositol was examined in two-cell and four-cell embryos (day 2 after insemination), morulae (day 6) and early blastocysts (day 7). Uptake in all stages examined was largely sodium-dependent indicating the presence of a sodium-dependent inositol transporter. Uptake of inositol did not vary significantly from two-cell to early blastocyst stages when expressed either on a per embryo or a per g of protein basis. Incorporation of inositol into the three phosphoinositides, PtdIns, PtdInsP, and PtdInsP 2 , was detectable at all stages examined. In contrast, incorporation of inositol into inositol phosphates was not detected until blastocyst formation at day 7. The second messenger, Ins(1,4,5)P 3 , was first detected in day 7 blastocysts.