Uptake and assimilation of sulphate by sulphur deficient Zea mays cells: The role of O-acetyl-L-serine in the interaction between nitrogen and sulphur assimilatory pathways
✍ Scribed by David T Clarkson; Eugénio Diogo; Sara Amâncio
- Publisher
- Elsevier Science
- Year
- 1999
- Tongue
- English
- Weight
- 84 KB
- Volume
- 37
- Category
- Article
- ISSN
- 0981-9428
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✦ Synopsis
Cell suspension cultures of maize (Zea mays) growing on a modified Murashige and Skoog's (MS) medium containing 1/10 of the normal N supply, were subjected to SO 4 2-starvation for 4 d. During the period of the experiment, the batches of cells were growing at similar rates in both +S and -S treatments. S-starved cells (-S) took up SO 4 2-at eight to ten times the rate of S-sufficient (+S) cells. The high uptake rate of -S cells was repressed within 1-2 h after SO 4 2-was re-supplied. The response to S-starvation was strongly diminished in cells which had been deprived of a N-source for 4 d. Cells grown for several culture cycles with homocysteine thiolactone (TL) as sole S-source had greatly increased SO 4 2-uptake rates. This enhanced uptake was repressed at similar rates by provision of SO 4 2-or by the renewal of the TL supply. The latter result was unexpected and cannot be explained at present. ATP-sulphurylase (EC 2.7.7.4) activity was also de-repressed: in -S cells, the measured activity being more than four times that in +S cells. Repression by SO 4 2-was observed although after a longer period than that for the repression of SO 4 2-uptake. In +S cells, SO 4 2-uptake and ATP-sulphurylase activity were increased significantly by the addition of 0.5 mol⋅m -3 O-acetyl-L-serine to the culture. Simultaneously, the cysteine pool increased in the same proportion as the former activities. The addition of other amino acids, viz. glutamine or alanine, had either negative effects or no effect on SO 4 2-uptake. © Elsevier, Paris O-acetyl-L-serine / sulphate assimilation / sulphate uptake / sulphur-deficiency / Zea mays cells APS, adenosine phospho sulphate / HPLC, high performance liquid chromatography / MS, Murashige and Skoog's medium / OAS, O-acetyl-L-serine / TL, DL-homocysteine thiolactone