Upregulation of the BRCA1 gene in human germ cells and in preimplantation embryos
✍ Scribed by Sandrine Giscard d’Estaing; Delphine Perrin; Gilbert M. Lenoir; Jean François Guérin; Robert Dante
- Book ID
- 118445434
- Publisher
- Elsevier Science
- Year
- 2005
- Tongue
- English
- Weight
- 122 KB
- Volume
- 84
- Category
- Article
- ISSN
- 1556-5653
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The study of gene expression in human preimplantation embryos is establishing itself as a necessary dimension of developmental biology and medical genetics. Transcripts identified in human preimplantation embryos include housekeeping genes, transcription and growth factor genes, sex-determining gene
A competitive polymerase chain reaction has been developed for quantitation of BRCA1 mRNA. In human cancer cell lines, the amount of BRCA1 mRNA is relatively low, ranging from 6 to 38 copies per cell. The decay rate of these transcripts in actinomycin-treated cells indicates that the half-life of th