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Upregulation of interleukin-1 by Epstein–Barr virus latent membrane protein 1 and its possible role in nasopharyngeal carcinoma cell growth

✍ Scribed by Yu-Tzu Huang; Mei-Ying Liu; Ching-Hwa Tsai; Te-Huei Yeh

Publisher
John Wiley and Sons
Year
2009
Tongue
English
Weight
273 KB
Volume
32
Category
Article
ISSN
1043-3074

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✦ Synopsis

Abstract

Background

Nasopharyngeal carcinoma (NPC) is associated with Epstein–Barr virus (EBV) infection. We previously found that interleukin (IL)‐1α and IL‐1β significantly increased in NPC tissues. This study investigated what EBV‐encoded proteins were involved in such IL‐1 production.

Methods and Results

IL‐1α and IL‐1β messenger ribonucleic acids (mRNAs) were detected in the EBV latent membrane protein 1 (LMP1) transfectant (LMP135) only by reverse transcriptase–polymerase chain reaction (RT‐PCR). LMP1‐mediated IL‐1α and IL‐1β production could be enhanced by tumor necrosis factor alpha (TNF‐α), determined by enzyme‐linked immunosorbent assay (ELISA). Moreover, IL‐1α and IL‐1β mRNAs and proteins were increased in a dose‐dependent manner in epithelial cells transiently transfected by an LMP1 plasmid. Besides, immortalized human epidermal keratinocyte (RHEK‐1) epithelial cells could be enhanced to proliferate by IL‐1α and IL‐1β determined by water‐soluble tetrazolium salt (WST‐1) assay.

Conclusions

EBV LMP1 is capable of upregulating IL‐1α and IL‐1β secretions from epithelial cells and positively modulated by TNF‐α. This may consequently contribute to tumor growth in patients with NPC. © 2009 Wiley Periodicals, Inc. Head Neck, 2009

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