Up-regulation of MSH receptors by MSH in cloudman melanoma cells
โ Scribed by Ashok K. Chakraborty; John M. Pawelek
- Book ID
- 115765359
- Publisher
- Elsevier Science
- Year
- 1992
- Tongue
- English
- Weight
- 679 KB
- Volume
- 188
- Category
- Article
- ISSN
- 0006-291X
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Expression of internal receptors for MSH is an important criterion for responsiveness to MSH by Cloudman melanoma cells (Orlow et al: ), Cell. Physiol., 142:129-I 36, 1990). Here, we show that internal and external receptors for MSH are of identical molecular weights (50-53 kDa) and share common ant
Cloudman S91 mouse melanoma cells express both external (plasma membrane) and internal binding sites for MSH. Using '"I-beta melanotropin (P-MSH) as a probe, we report here an extensive series of studies on the biological relevance of these internal sites. Cells were swollen in a hypotonic buffer an