✦ LIBER ✦
Unusually large electrostatic field effect of the buried aspartate in serine proteinases: Source of catalytic power
✍ Scribed by Gábor Náray-Szabó
- Book ID
- 104580914
- Publisher
- John Wiley and Sons
- Year
- 1983
- Tongue
- English
- Weight
- 315 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0020-7608
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✦ Synopsis
Abstract
Calculating the electrostatic potential around the Ser–His–Asp catalytic triad in serine proteinases the very important substituent effect of the buried aspartate is revealed. It is found that the strong Coulomb field of this distant but charged side chain considerably enhances the nucleophilicity of the active serine hydroxyl group. The interpretation of the vital importance of aspartate may replace the “charge–relay” hypothesis which seems to be disproved in the light of recent experiments.