Untersuchungen über ATP und andere organische Phosphorverbindungen im Siebröhrensaft vonYucca flaccidaundSalix triandra
✍ Scribed by Manfred Kluge; Ditmar Becker; Hubert Ziegler
- Publisher
- Springer-Verlag
- Year
- 1970
- Tongue
- English
- Weight
- 978 KB
- Volume
- 91
- Category
- Article
- ISSN
- 0032-0935
No coin nor oath required. For personal study only.
✦ Synopsis
Eingegangen am 21. November i969
Investigations on ATP and Other Organic Phosphorus Compounds in the Sieve Tube Sap of Yucca ]laccida and Salix triandra Summary. 1. Like the sieve tube sap of dicotyledons analysed so far, the phloem exudate of Yucca [laccida contains a high level of ATP, i.e. 615 ~g/ml on the average. ATP concentration in the phloem exudate does not decrease during extended periods of exudation.
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The pattern of labelled phosphorylated compounds after feeding" of ~2PO~-is essentially similar in the phloem exudate of Yucca [laccida and in the exudate from the mouth parts of aphids feeding on Salix triandra phloem.
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The label was incorporated very rapidly into most of the phosphorylated organic compounds present in the exudate of Yucca /laccida. In the first phase after feeding of a~PO~--only the label in fructose-l,6-diphosphate and possibly the label in ATP was still increasing in relation to the total radioactivity. This increment was correlated to a loss of radioactivity in the inorganic phosphate fraction.
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Specific radioactivity of inorganic phosphate and ATP in the sieve tube sap of Yucca ]Iaccida increased continuously throughout the experiments while a2POi--was administered externally. This trend continued for some time after removal of the external source of label.
Einleitung
Den Phosphorverbindungen in den SiebrShren wurde in den letzten Jahren zunehmende Aufmerksamkeit gesehenkt. Es stellte sich heraus, dal~ der SiebrShrensaft neben dem inengenm~gig weir dominierenden Phosphat (vgl. Ziegler, 1956) noeh eine Reihe anderer Phosphorverbindungen enthi~lt, yon denen das ATP wegen seiner relativ hohen Konzentration (Kluge u. Ziegler, 1964; Gardner u. Peel, 1969) besonderes Interesse verdient. Durch Verwendung yon radioaktivem Phosphor konute Bieleski (1969) zeigen, dab bei Cucurbita maxima das ATP und andere organische Phosphorverbindungen (wie z.B. Hexosemonophosphate und UDPG) im Phloemexsudat einen lebhaften Phosphataustauseh mit dem anorganisehen Phosphat aufweisen. Er kam zu dem Sehlug, dal] die Siebr6hren von Cucurbita metaboliseh aktiv seien und * Herrn Prof. Dr. Dr. h. c. Bruno Huber, lV[iinchen, zum 70. Geburtstag gewidmet.