Untersuchungen zur Antigenstruktur von Shigellen Heterogenität der spezifischen Polysaccharide von zwei Shigella flexneri-Stämmen und zwei Sh. flexneri/Escherichia coli-Hybriden

✍ Scribed by Dr. G. Seltmann; W. Beer

• Book ID: 102909814
• Publisher: John Wiley and Sons
• Year: 2007
• Tongue: English
• Weight: 584 KB
• Volume: 17
• Category: Article
• ISSN: 0233-111X
• DOI: 10.1002/jobm.19770170508

✦ Synopsis

Abstract

The S‐specific polysaccharide from 2 Sh. flexneri wild strains (with serological var. X‐ and var. Y‐specificity, respectively) and 2 Sh. flexneri E. coli hybrids (with the same specificities) can be separated by means of gel chromatography on Sephadex G‐200 and G‐50 into altogether 6 fractions per strain. Fraction G‐200/1 (molecular weight >10^6^D) represents a polymer consisting nearly exclusively of glucose and is present mainly in the two Y‐type strains, much less in the two X‐type strains. Fractions G‐200/2 and G‐200/3 (molecular weight ∼ 10^5^D and ∼ 2 · 10^4^ D, respectively) seem to consist mainly of the S‐specific side chains while fraction G‐50/2 (molecular weight ∼ 2000 D) presumably contains an SR‐polysaccharide (core with one repeating unit). Fraction G‐50/3 (molecular weight ∼ 1000 D) contains the core polysaccharide and fraction G‐50/4 splitting products (mainly KDO). No significant differences in chromatographical behaviour and quantitative composition could be found between the polysaccharides of the wild strains and the hybrid strains

Because of the well‐known stability of the glucosaminyl linkages the sugar analysis was not only performed after acidic hydrolysis. In some cases the acid hydrolysate was reacted with HNO~2~ to cleave the glucosaminyl linkages. In most cases the values obtained now were higher than those obtained directly.