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Universal Labeling Chemistry for Nucleic Acid Detection on DNA-Arrays

โœ Scribed by E. Bernal-Mendez; C. Tora; I. Sothier; M. Kotera; A. Troesch; A. Laayoun


Publisher
John Wiley and Sons
Year
2004
Weight
54 KB
Volume
35
Category
Article
ISSN
0931-7597

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Biotinylation of DNA on Membrane Support
โœ V.V. Didenko ๐Ÿ“‚ Article ๐Ÿ“… 1993 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 389 KB

We have used M13 single-stranded DNA bound by uv to small pieces of nylon membrane for the synthesis of biotinylated single-stranded DNA probes. The labeling method requires a large fragment of DNA polymerase I and random hexanucleotides. There is no need for previous linearization of the template.