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Unfolding and Degradation of Proteins by ClpXP Monitored with Single Molecule FRET

✍ Scribed by van Ginkel, Jetty; Meyer, Anne; Joo, Chirlmin

Book ID
122240199
Publisher
Biophysical Society
Year
2013
Tongue
English
Weight
64 KB
Volume
104
Category
Article
ISSN
0006-3495

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## Abstract Single‐molecule FΓΆrster resonance energy transfer (FRET) measurements with phosphoglycerate kinase from yeast were performed at different concentrations of guanidine hydrochloride. From these steady‐state measurements we obtained FRET efficiency histograms characterizing structural prop