A simple method for the purification of
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David Konecki; J. Miguel Cimadevilla; Gisela Kramer; Boyd Hardesty
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Article
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1975
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Springer
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English
โ 330 KB
A relatively simple and inexpensive method has been developed for the preparation of highly purified rabbit reticulocyte globin mRNA. After phenol extraction, polysomal RNA was chromatographed on Sigmacell type 38 cellulose and Sepharose 4B. The resulting mRNA preparation has a purity in excess of 9