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Unexpected Reactivity of the Burgess Reagent with Thiols: Synthesis of Symmetrical Disulfides

โœ Scribed by Banfield, Scott C.; Omori, Alvaro Takeo; Leisch, Hannes; Hudlicky, Tomas


Book ID
118179279
Publisher
American Chemical Society
Year
2007
Tongue
English
Weight
80 KB
Volume
72
Category
Article
ISSN
0022-3263

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The synthesis of difluorescein disulfide, a new fluorescent thiol-specific reagent, is described. The absorption and fluorescence spectra and pH dependency of this compound and of several other fluorescein derivatives are presented. Histone H3 labeled with difluorescein disulfide displays a high flu