Understanding the dynamics of cellular responsiveness to modifications of metabolic substrates in perifusion

✍ Scribed by Dr. Rhonda M. Brand; R. H. Lyons; A. Rees Midgley

• Book ID: 102885150
• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 806 KB
• Volume: 160
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041600103

✦ Synopsis

A novel microperifusion system with capabilities for continuous, real-time, potentiometric monitoring of extracellular hydrogen ion concentration has been used to define the response of HeLa cells to abrupt changes in extracellular energy sources or introduction of an inhibitor of glycolysis. Glycolytic inhibition, induced by removal of glucose or introduction of iodoacetate, each led to a rapid, continuous decrease in acid release. The response to iodoacetate took longer than removal of glucose, perhaps due to the time required for binding and activation. Once inhibition began, however, the rate of change was greater than following glucose removal. Conversely, recovery time following iodoacetate inhibition was much slower than with glucose removal. Unlike the response to short-term glucose depletion, a second pulse of iodoacetate resulted in a faster response followed by an even longer recovery time. The response to switching between glucose and glutamine began almost without evident delay. The response patterns