Unconventional immobilization of dextransucrase with alginate
โ Scribed by Astrid Reischwitz; Klaus-Dieter Reh; Klaus Buchholz
- Publisher
- Elsevier Science
- Year
- 1995
- Tongue
- English
- Weight
- 602 KB
- Volume
- 17
- Category
- Article
- ISSN
- 0141-0229
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## Abstract Dextransucrase from __Leuconostoc mesenteroides__ (NRRL Bโ512F) was purified by ultrafiltration and gel filtration chromatography in 54% yield. The specific activity of a heart cut was 58.6 U/mg; cumulative purification of that preparation was 247โfold. Of 13 carriers surveyed, only alk
## Abstract The porosity of Shirasu Porous Glass (SPG) membranes was controlled continuously with dextran produced by the enzymatic reaction of dextransucrase (DSase). DSase was immobilized inside the pores of an SPG membrane at a density of 1.5 U g^โ1^, and a sucrose solution was subsequently perm