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Uncharged tRNA inhibits guanosine 3′,5′-bis (diphosphate) 3′-pyrophosphohydrolase [ppGppase], the spoT gene product, from Escherichia coli

✍ Scribed by Richter, Dietmar

Book ID: 104694934
Publisher: Springer
Year: 1980
Tongue: English
Weight: 246 KB
Volume: 178
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00270479

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✦ Synopsis

The spot gene product from Escherichia coli, the guanosine 3',5'-bis(diphosphate) 3'-pyrophos phohydrolase [ppGppase] catalyzes the specific release of pyrophosphate from the 3'-position of guanosine 3',5'-bis(diphosphate) [ppGpp]; this reaction is significantly inhibited in the presence of uncharged tRNAPhe yeast. Little or no inhibition is observed with Phe-tRNAPhe, tRNAPhe-CpCpAoxi-red or ribosomal RNA (16S and 23S).

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