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Unambiguous identification of histone H1 in Trypanosoma cruzi

✍ Scribed by G. Cecilia Toro; Norbel Galanti; Ulf Hellman; Christer Wernstedt

Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
767 KB
Volume
52
Category
Article
ISSN
0730-2312

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✦ Synopsis

The existence of histone HI has been questioned in Trypanosomatids. We report here the presence of a histone HI in the chromatin of Trypanosoma cruzi. This protein was purified by narrow-bore reversed phase HPLC and its amino acid composition analyzed and compared with histones HI from other species. Furthermore, the purified chromosomal protein was digested with proteases and the amino acid sequences of the resulting peptides were analyzed by the automated Edman degradation. The sequences obtained were found to present a high degree of homology when compared to the carboxy terminal domain of other known histones HI.

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