Ultraweak light emission is a common response of bacterial cells to chemico-physical stress
✍ Scribed by Maccarrone, Mauro ;Agro′, Alessandro Finazzi ;Rosato, Nicola
- Publisher
- John Wiley and Sons
- Year
- 1998
- Weight
- 141 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0884-3996
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✦ Synopsis
Escherichia coli JM101 cells were subjected to pore-forming electric ®elds, irradiation with ultraviolet light or oxidative stress by either the lipoxygenase products 9-and 13hydroperoxyoctadecadienoic acids (9-and 13-HPOD) or hydrogen peroxide. It was found that all chemico-physical stresses enhanced ultraweak light emission from the bacterial cells, the most effective treatment being electroporation (up to 20-fold increase in luminescence compared to the control value), followed by oxidative stress with 9-or 13-HPOD (up to 4-fold increase) and irradiation with UV light (up to 2.8-fold increase). Bacterial luminescence was always in the red edge of the spectrum and was paralleled by changes in membrane oxidative index and speci®c activity of catalase and superoxide dismutase.