Ultraviolet circular dichroism of polyproline and oriented collagen

✍ Scribed by Richard Mandel; George Holzwarth

• Book ID: 102760289
• Publisher: Wiley (John Wiley & Sons)
• Year: 1973
• Tongue: English
• Weight: 991 KB
• Volume: 12
• Category: Article
• ISSN: 0006-3525
• DOI: 10.1002/bip.1973.360120317

✦ Synopsis

Abstract

The ultraviolet absorption, linear dichroism, circular dichroism, and oriented circular dichroism of collagen are reported and the spectra are resolved into a self‐consistent set of bands in accord with exciton theory. The parallel band at 200 nm has 40% of the π → π* intensity; the perpendicular band is placed at 189 nm yielding a splitting of 2700 cm^−1^. The circular dichroism is resolved into two Gaussians at λ~∥~ and λ~τ~ (rotational strengths +14 × 10^−40^ and −32 × 10^−40^ esu^2^. cm^2^) plus a large non‐Gaussian (“helix”) band with ampplitude −25,000° at 201 nm. These data appear to be in reasonably good accord with recent calculations.

Measurements of the absorption, linear dichroism and circular dichroism of polyproline I and II are also reported and are resolved into their component bands. Polyproline I is in good accord with exciton theory, whereas polyproline II remains unsatisfactory.