𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Ultraviolet B radiation activates NF-κB and induces iNOS expression in HR-1 hairless mouse skin: Role of IκB kinase-β

✍ Scribed by Eun-Jin Chang; Joydeb Kumar Kundu; Lijia Liu; Jun-Wan Shin; Young-Joon Surh

Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
706 KB
Volume
50
Category
Article
ISSN
0899-1987

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Exposure to ultraviolet B (UVB) radiation is known to cause inflammatory tissue damage and skin cancer. One of the molecular links between inflammation and cancer is the eukaryotic transcription factor nuclear factor‐kappaB (NF‐κB), which is known to regulate expression of various pro‐inflammatory genes including inducible nitric oxide synthase (iNOS). The present study was aimed at elucidating the molecular mechanisms underlying UVB‐induced NF‐κB activation and iNOS expression in hairless mouse skin. Irradiation of male HR‐1 hairless mouse skin with UVB (5 kJ/m^2^) resulted in increased degradation of IκBα, nuclear translocation of p65 and p50, and the DNA binding of NF‐κB. Exposure to UVB radiation induced the phosphorylation and the catalytic activity of an upstream kinase IκB kinase‐β (IKKβ). Pharmacological inhibition of IKKβ attenuated UVB‐induced NF‐κB activation in mouse skin. Irradiation of mouse skin with UVB also increased phosphorylation of extracellular signal‐regulated kinase (ERK) and p38 mitogen‐activated protein (MAP) kinase. Pretreatment with SC‐514, a specific inhibitor of IKKβ, attenuated UVB‐induced phosphorylation of ERK and p38 MAP kinase. A kinetic study showed that UVB significantly increased the expression of iNOS in mouse skin at 6 h postirradiation, which was abrogated by pretreatment with SC‐514. In conclusion, the upstream kinase IKKβ is involved in UVB‐induced activation of MAP kinases and NF‐κB, and expression of iNOS in mouse skin. © 2011 Wiley‐Liss, Inc.

📜 SIMILAR VOLUMES

Differential roles of hydrogen peroxide
Differential roles of hydrogen peroxide and superoxide in mediating IL-1-induced NF-κB activation and iNOS expression in bovine articular chondrocytes
✍ Alexandrina Ferreira Mendes; M. Margarida Caramona; A. Pato Carvalho; M. Celeste 📂 Article 📅 2003 🏛 John Wiley and Sons 🌐 English ⚖ 259 KB

## Abstract Our previous studies showed that reactive oxygen species (ROS) are required for the pro‐inflammatory cytokine interleukin‐1β (IL‐1) to induce the activity of the Nuclear transcription Factor‐kappaB (NF‐κB) and the expression of the inducible isoform of the nitric oxide synthase (iNOS) i

Cytokine-stimulated inducible nitric oxi
Cytokine-stimulated inducible nitric oxide synthase expression in astroglia: Role of Erk mitogen-activated protein kinase and NF-κB
✍ Joshua S. Marcus; Sharon L. Karackattu; Melissa A. Fleegal; Colin Sumners 📂 Article 📅 2002 🏛 John Wiley and Sons 🌐 English ⚖ 308 KB 👁 1 views

## Abstract Expression of inducible nitric oxide synthase (iNOS), which leads to the production of nitric oxide (NO), is stimulated by proinflammatory cytokines such as interleukin‐1β (IL‐1β) and tumor necrosis factor‐α (TNF‐α). Here we report on the roles of nuclear factor‐κB (NF‐κB) and mitogen‐a

Role of atypical protein kinase C isozym
Role of atypical protein kinase C isozymes and NF-κB in IL-1β-induced expression of cyclooxygenase-2 in human myometrial smooth muscle cells
✍ Sara V. Duggan; Tamsin Lindstrom; Teresa Iglesias; Phillip R. Bennett; Giovanni 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 230 KB

## Abstract Increased myometrial expression of cyclooxygenase‐2 (Cox‐2) at term results from elevated local levels of inflammatory cytokines, and its inhibition provides a potential route for intervention in human pre‐term labor. We have identified a role for atypical protein kinase C (PKC) isozyme

Glucosamine inhibits LPS-induced COX-2 a
Glucosamine inhibits LPS-induced COX-2 and iNOS expression in mouse macrophage cells (RAW 264.7) by inhibition of p38-MAP kinase and transcription factor NF-κB
✍ Mohamed M. Rafi; Prem N. Yadav; Andrea O. Rossi 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 774 KB

## Abstract Glucosamine supplements are very promising nonsteroidal anti‐inflammatory agents widely used for the treatment of arthritis in animals and humans. In this study, we have proposed the molecular mechanism underlying the anti‐inflammatory properties of glucosamine hydrochloride (GLN) using