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Ultrathin-Layer Zone Electrophoresis of Lactate Dehydrogenase Isoenzymes in Microdissected Liver Samples

✍ Scribed by I.P. Maly; M. Toranelli

Publisher: Elsevier Science
Year: 1993
Tongue: English
Weight: 664 KB
Volume: 214
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.1993.1512

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✦ Synopsis

A highly sensitive electrophoretic separation of lactate dehydrogenase isoenzymes in 150-microns ultrathin-layer polyacrylamide gels is described. By means of the incorporation of miniature-sized wells into the gel and by executing all the analytical steps under paraffin oil, this technique allows the exact and sharp separation of the lactate dehydrogenase isoenzymes in nanogram-sized microdissected liver tissue samples. With this method the heterogeneous distribution pattern of lactate dehydrogenase isoenzymes in the liver acinus of various mammals could be demonstrated, and a new interpretation of their functional roles is offered.

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