Ultrastructures for one- or two-dimensional confinement in compound semiconductors

In-gel digestion of proteins for interna

In-gel digestion of proteins for internal sequence analysis after one- or two-dimensional gel electrophoresis

✍ Jorge Rosenfeld; Joël Capdevielle; Jean Claude Guillemot; Pascual Ferrara 📂 Article 📅 1992 🏛 Elsevier Science 🌐 English ⚖ 940 KB

We examined the different steps necessary for the enzymatic digestion of proteins in the polyacrylamide matrix after gel electrophoresis. As a result, we developed an improved method for obtaining peptides for internal sequence analysis from 1-2 micrograms of in-gel-digested proteins. The long washi