Ultrastructure and assembly of segmental long spacing collagen studied by atomic force microscopy

✍ Scribed by M.F Paige; M.C Goh

• Book ID: 104368952
• Publisher: Elsevier Science
• Year: 2001
• Tongue: English
• Weight: 611 KB
• Volume: 32
• Category: Article
• ISSN: 0968-4328
• DOI: 10.1016/s0968-4328(00)00038-x

✦ Synopsis

The in vitro formation of segmental long spacing (SLS) collagen as induced by the addition of ATP to acidified Type I collagen solutions has been examined with the atomic force microscope (AFM). AFM images obtained suggest that the assembly proceeds in a stepwise manner, through an intermediate stage of oligomers, which then associate laterally to form the so-called "SLS crystallites". Attempts to induce SLS formation by the addition of other polyanionic species to monomeric collagen solutions met with mixed success; ATP-gamma-S and GTP produced SLS crystallites, whereas inorganic phosphate and other polyanionic dyes did not. This indicates that the formation of SLS cannot simply be attributed to the negation of positive charges believed to be located on the end of the collagen monomer, but rather it is a complex function of the structure and charge of both the collagen monomer and polyanion.