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Ultrastructural Studies of Fibroblasts Transfected with Hepatitis B Virus DNA

✍ Scribed by Naoto Aoki; Michael A. Gerber; Swan N. Thung; Mei-Ling Chen; Judith K. Christman; Peter M. Price; Christos S. Flordellis; George Acs

Book ID: 102848328
Publisher: John Wiley and Sons
Year: 1984
Tongue: English
Weight: 807 KB
Volume: 4
Category: Article
ISSN: 0270-9139
DOI: 10.1002/hep.1840040115

No coin nor oath required. For personal study only.

✦ Synopsis

Cultured 3T3 mouse fibroblasts transfected with cloned hepatitis B virus genome and DNA coding for methotrexate-resistant dihydrofolate reductase, produce and secrete significant amounts of hepatitis B surface antigen (HBsAg). Ultrastructural morphometry revealed that fibroblasts transfected with hepatitis B virus DNA contained significantly more lysosomes than did fibroblasts transfected with the gene coding for methotrexate resistance or normal fibroblasts. Although abundant HBsAg was found in the cytoplasm of transfected fibroblasts by immunologic methods, HBsAg particles were not detected by electron microscopy. Immunoelectron microscopy localized HBsAg to the nuclear envelope, rough endoplasmic reticulum, and endoplasmic cisternae. These findings suggest that the transfected cells produce mainly nonparticulate HBsAg or that they have a defect in intracisternal packaging of HBsAg into particles.

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