Human hepatocyte growth factor stimulates DNA synthesis by cultured rat hepatocytes. When human hepatocyte growth factor prepared from the culture medium of human embryonic lung fibroblasts was intravenously injected into normal rats and rats after 70% hepatectomy, it was detected in hepatocytes but
Ultrastructural location of human hepatocyte growth factor in human liver
β Scribed by Hiroki Sakaguchi; Shuichi Seki; Hirohito Tsubouchi; Yasushi Daikuhara; Yoshiyuki Niitani; Kenzo Kobayashi
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- English
- Weight
- 768 KB
- Volume
- 19
- Category
- Article
- ISSN
- 0270-9139
No coin nor oath required. For personal study only.
β¦ Synopsis
for critical comments and helpful discussions, and Dr. Tadashi Hishida, Research Center, Mitsubishi Kasei Corp., Yokohama, for supplying recombinant hHGF. We also thank Drs. Nobuyoshi Kawakita and Atsushi Yanai for technical assistance and helpful discussions.
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The effect of recombinant human hepatocyte growth factor (h-rHGF), a potent mitogen for hepatocytes, was investigated in primary cultures of human hepatocytes. Here, we describe a series of experiments to investigate the kinetics of its mitogenic action, as well as its metabolic effects on cultured
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## Abstract The effect of hepatocyte growth factor/scatter factor (HGF/SF) on the proliferation of human skin fibroblasts was examined. At concentrations above 1.0 ng/ml, both native and recombinant human HGF/SF stimulated the DNA synthesis determined by [^3^H]thymidine incorporation, which was com