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Ultrastructural localization of orexin-1 receptor in pre- and post-synaptic neurons in the rat arcuate nucleus

✍ Scribed by Jian-Lian Guan; Ryusuke Suzuki; Hisayuki Funahashi; Qing-Ping Wang; Haruaki Kageyama; Kichio Uehara; Shuori Yamada; Shinobu Tsurugano; Seiji Shioda


Book ID
117472999
Publisher
Elsevier Science
Year
2002
Tongue
English
Weight
280 KB
Volume
329
Category
Article
ISSN
0304-3940

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A simple pre-embedding avidin-biotin-peroxidase complex technique was used to study the ultrastructural localization of mu-1 opioid receptor in the rat dorsal raphe nucleus. Using low concentrations of the first antiserum for incubation with a short reaction time to 3,3'-diaminobenzidine, the immuno