Ultrastructural evidence of calcium uptake by chloride cells in the gills of goldfish,Carassius auratus
✍ Scribed by Ishihara, Akiko ;Mugiya, Yasuo
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- English
- Weight
- 944 KB
- Volume
- 242
- Category
- Article
- ISSN
- 0022-104X
No coin nor oath required. For personal study only.
✦ Synopsis
Calcium uptake by the gills in goldfish (Curussius uurutus) was observed by electron microscopy using an oxalate-based method which specifically induced electron-opaque precipitates in association with chloride cells in the primary epithelium. EDTA treatment and x-ray microanalysis substantiated that these precipitates are a calcium salt. They were never found in deionized-water-adapted fish. Oxalate-induced calcium precipitates, varying from 0.04 to 0.2 pm in diameter, occurred in the apical crypts of chloride cells and in large vacuole-like structures in the lateral and basolateral regions of the cells. The crypts where the precipitates arose scatteringly had the morphological characteristics similar to the normal morphology of apical crypts: they were 2 to 4 pm in diameter and lined with darker and homogeneous cytoplasm with microridges. The vacuole-like structures containing numerous calcium precipitates were somewhat irregular in contour and had a diameter of about 3 pm. They appeared to contact a densely distributed tubular system, but their limiting membrane was uncertain in the present resolution. The precipitates were concentrated in the central region of the structures. These results suggest that branchial calcium uptake is closely associated with chloride cells in goldfish.