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Ultrasonic absorption of DNA solutions: influence of pH

โœ Scribed by Jean Sturm; Jacques Lang; Raoul Zana


Publisher
Wiley (John Wiley & Sons)
Year
1971
Tongue
English
Weight
316 KB
Volume
10
Category
Article
ISSN
0006-3525

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โœฆ Synopsis


Recently one of us has shown that the ultrasonic absorption of DNA solutions goes through a well-defined maximum when the pH is changed.1 This maximum occurs, independently of the ultrasonic frequency, at pH = 11.9, that is, at a pH close to that which corresponds to the mid-point of the denaturation of the DNA molecule, as found by optical rotatory dispersion measurements' for instance. It was then suggested that this maximum might be associated with the perturbation by the sound wave of some equilibria between the native and denatured forms of the DNA molecule. Indeed, Schwarz's theory2 predicts such a behavior of t,he ultrasonic absorption when the molerules in solution undergo a conformal change induced for instance by a pH variation.


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