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Ultrasensitive immune complex transfer enzyme immunoassay of HIV-1 p24 antigen with less serum interference using 2,4-dinitrophenyl-anti-HIV-1 p24 IgG and indirectly immobilized (anti-2,4-dinitrophenyl group) fab′

✍ Scribed by Setsuko Ishikawa; Seiichi Hashida; Kazuya Hashinaka; Atsushi Saito; Akihisa Takamizawa; Hideo Shinagawa; Eiji Ishikawa

Publisher
John Wiley and Sons
Year
1999
Tongue
English
Weight
55 KB
Volume
13
Category
Article
ISSN
0887-8013

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Ultrasensitive and more specific enzyme
Ultrasensitive and more specific enzyme immunoassay (immune complex transfer enzyme immunoassay) for p24 antigen of HIV-1 in serum using affinity-purified rabbit anti-p24 Fab′ and monoclonal mouse anti-p24 Fab′
✍ Seiichi Hashida; Kazuya Hashinaka; Ichiro Nishikata; Atsushi Saito; Akihisa Taka 📂 Article 📅 1996 🏛 John Wiley and Sons 🌐 English ⚖ 517 KB

Previously, an ultrasensitive enzyme immunoassay (immune complex transfer enzyme immunoassay) for p24 antigen of HIV-1 was developed. The immune complex comprising 2,4-dinitrophenyl-biotinyl-bovine serum albumin-rabbit anti-p24 Fab' conjugate, p24 antigen, and rabbit anti-p24 Fab'-p-Dgalactosidase c