Ultrasensitive Hydrogen Peroxide Biosensor Based on Enzyme Bound to Layered Nonoriented Multiwall Carbon Nanotubes/Polyelectrolyte Electrodes
✍ Scribed by Bernard S. Munge; Richard S. Dowd; Colleen E. Krause; Lines N. Millord
- Publisher
- John Wiley and Sons
- Year
- 2009
- Tongue
- English
- Weight
- 321 KB
- Volume
- 21
- Category
- Article
- ISSN
- 1040-0397
No coin nor oath required. For personal study only.
✦ Synopsis
Abstract
A sensitive hydrogen peroxide sensor based on horseradish peroxidase covalently attached to layered nonoriented MWNTs modified electrode is presented. Cyclic voltammetry results gave quasi‐reversible Fe^III^/Fe^II^ voltammetry. The electron transfer rate constant (k~s~) and Michaelis–Menten constant (K~M~) in pH 7 is 48.8±0.9 s^−1^ and 0.13±0.05 mM respectively. A linear calibration curve for hydrogen peroxide was obtained up to 120 nM under the optimized conditions with a remarkable detection limit of (S/N=3) 1.5 nM. Results suggest that the nonoriented nanotubes act as electrical conductors and may also provide large surface area facilitating facile electron transfer and excellent electrochemical catalysis.