Ultrasensitive Detection of Low-Abundance Surface-Marker Protein Using Isothermal Rolling Circle Amplification in a Microfluidic Nanoliter Platform
✍ Scribed by Tania Konry; Irina Smolina; Joel M. Yarmush; Daniel Irimia; Martin L. Yarmush
- Book ID
- 104593613
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 509 KB
- Volume
- 7
- Category
- Article
- ISSN
- 1613-6810
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✦ Synopsis
With advances in immunology and cancer biology, there is an unmet need for increasingly sensitive systems to monitor the expression of specifi c cell markers for the development of new diagnostic and therapeutic tools. To address this challenge, a highly sensitive labeling method that translates antigen-antibody recognition processes into DNA detection events that can be greatly amplifi ed via isothermal rolling circle amplifi cation (RCA) is applied. By merging the single-molecule detection power of RCA reactions with microfl uidic technology, it is demonstrated that the identifi cation of specifi c protein markers can be achieved on tumor-cell surfaces in miniaturized nanoliter reaction droplets. Furthermore, this combined approach of signal amplifi cation in a microfl uidic format could extend the utility of existing methods by reducing sample and reagent consumption and enhancing the sensitivities and specifi cities for various applications, including early diagnosis of cancer.