Ultramicro-electrophoresis of protein in sweat from patients with cystic fibrosis of the pancreas and controls

✍ Scribed by Claus-P. Schröder; Hinderk M. Emrich

• Publisher: Springer
• Year: 1978
• Tongue: English
• Weight: 527 KB
• Volume: 128
• Category: Article
• ISSN: 0340-6997
• DOI: 10.1007/bf00496926

✦ Synopsis

46 sweat-samples from 32 children with cystic fibrosis of the pancreas (C.F.) and 35 samples from 23 control-children were collected with glass micro-capillaries. Protein was determined by ultramicro-electrophoresis.

Results:

1. protein was detected in 48% of the samples (c.f. and controls). 2. at ph 2.3, c.f.-sweat showed at least one more band than control-sweat. 3. at ph 8.9, c.f.-sweat occasionally showed one more band than the control-group. 4. at ph 2.3, more protein was found at the electrophoresis start point using c.f.-sweat, whereas at ph 8.9 the opposite was found. 5. at ph 2.3, protein was found in fewer samples than at ph 8.9. the "c.f.-factor" is postulated to represent a basic polyelectrolyte which induces the following pathogenic mechanisms: a) aggregation of proteins giving rise to a high viscosity of secretions, such as saliva. b) binding to the cell-membrane of the glandular epithelium, thus inducing a disturbance of active nacl-reabsorption (e.g. by reduction of luminal passive na+-influx).