Ultrafiltration of fermented broths and solvent extraction of antibiotics

✍ Scribed by A. M. A. Nabais; J. P. Cardoso

• Book ID: 104742877
• Publisher: Springer
• Year: 1995
• Tongue: English
• Weight: 584 KB
• Volume: 13
• Category: Article
• ISSN: 1615-7605
• DOI: 10.1007/bf00367257

✦ Synopsis

Experiments of ultrafiltration with diafiltration were performed on fermented broths of benzylpenicillin and clavulanic acid produced at pilot scale at CIPAN, SA to achieve solid/liquid separation, antibiotic recovery and soluble protein retention. This study was done using a laboratorial/pilot scale ultrafiltration facility from Paterson Candy International Ltd. whose modules have a tubular configuration. PVDF membranes with a molecular weight cut-off of 100 000 (FP10) and 200 000 (FP200) were assessed. Comparison between solvent extraction of benzylpenicillin and clavulanic acid from filtered broth and ultrafiltered broth was also done. It was concluded that ultrafiltration with diafiltration of fermented broths of antibiotics using the FP10 membrane is a valid alternative to the use of rotary vacuum filters, flocculants and filter aid, deserving a further study to establish the best operating conditions. An economic appraisal also seems to be of paramount importance.

List of symbols CF

2 Mass WB g Pi bar RBv % RcA % CJ{SolPro t o,~ volumetric concentration factor concentration average permeate flux permeate flux at time t mass of fermented broth inlet pressure of feed benzylpenicillin recovered by mass balance to the permeate, defined by equation (4) clavulanic acid recovered by mass balance to the permeate, defined by equation (4) retained soluble protein by mass balance to the permeate, defined by equation (5)