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Ultrafiltration affinity purification : Isolation of concanavalin a from seeds of Canavalia Ensiformis

โœ Scribed by Bo Mattiasson; Matts Ramstorp


Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
611 KB
Volume
283
Category
Article
ISSN
1873-3778

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Leaching of concanavalin A during affini
โœ Yasmin Marikar; Bobby Zachariah; Debkumar Basu ๐Ÿ“‚ Article ๐Ÿ“… 1992 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 626 KB

Preparation of surface glycoproteins from human fetal brain cells by affinity chromatography on Con A-Sepharose 4B was a problematic endeavor due to leaching of Con A from the matrix. Dissociation of Con A from the matrix took place irrespective of the presence of lipid and/or detergent and the buff