Ultradian oscillation of Saccharomyces cerevisiae during aerobic continuous culture: hydrogen sulphide mediates population synchrony

✍ Scribed by Ho-Yong Sohn; Douglas B. Murray; Hiroshi Kuriyama

• Publisher: John Wiley and Sons
• Year: 2000
• Tongue: English
• Weight: 124 KB
• Volume: 16
• Category: Article
• ISSN: 0749-503X
• DOI: 10.1002/1097-0061(20000930)16:13<1185::aid-yea619>3.0.co;2-w

✦ Synopsis

Saccharomyces cerevisiae showed an ultradian respiratory oscillation during aerobic continuous culture. Analysis of the off-gas revealed that hydrogen sulphide production also oscillated. Production was ®rst detected at the onset of low respiration and reached a maximum (1.5 mM) prior to minimum respiratory activity. Then H 2 S concentration fell rapidly to below 0.2 mM before the onset of high respiration. Injection of respiratory oscillation perturbation agents, such as glutathione (50 mM), NaNO 2 (50 mM) or acetaldehyde (4.5 mM), transiently increased H 2 S production above 6 mM. The synchronization properties of H 2 S were analysed to reveal that changes of oscillation period and amplitude were dependent on H 2 S concentration in culture. It is concluded that H 2 S produced during oscillation produces population synchrony by respiratory chain inhibition.