Ubiquitous expression of mRFP1 in transgenic mice

✍ Scribed by Huaxing Zhu; Guangxue Wang; Gang Li; Min Han; Tian Xu; Yuan Zhuang; Xiaohui Wu

• Publisher: John Wiley and Sons
• Year: 2005
• Tongue: English
• Weight: 518 KB
• Volume: 42
• Category: Article
• ISSN: 1526-954X
• DOI: 10.1002/gene.20129

✦ Synopsis

Abstract

Fluorescent proteins provide a powerful means to track gene expression and cellular behaviors in the study of model organisms such as mice. Among the new generation of fluorescent protein markers, the monomeric red fluorescent protein mRFP1 is particularly attractive because of its rapid maturation and minimal interference with GFP and GFP‐derived markers. Here we evaluate the utility of mRFP1 as a marker in transgenic mice. We show that high level and ubiquitous expression of mRFP1 does not affect mouse development, general physiology, or reproduction. mRFP1 expression can be readily detected with unaided eyes under daylight in transgenic mice on the albino background. The intensity of mRFP1 signals can be used to distinguish homozygous and heterozygous transgenic mice. Together, these features make mRFP1 an attractive marker for broad applications in transgenic research. genesis 42:86–90, 2005. © 2005 Wiley‐Liss, Inc.