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Ubiquitination of an artificial RING finger without a substrate and a tag

✍ Scribed by Kazuhide Miyamoto

Book ID
105360050
Publisher
John Wiley and Sons
Year
2011
Tongue
English
Weight
193 KB
Volume
18
Category
Article
ISSN
1075-2617

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✦ Synopsis

Alpha‐helical region substitution was applied to the SIAH1 and EL5 RING fingers. The Williams–Beuren syndrome transcription factor (WSTF) PHD_SIAH1 and WSTF PHD_EL5 RING fingers were created as the artificial ubiquitin‐ligating enzyme (E3). These fingers possess E3 activities of mono‐ubiquitination and poly‐ubiquitination, respectively, with ubiquitin‐conjugating enzyme (E2)‐binding capabilities. Artificial E3s bind two zinc atoms and adopt a zinc‐dependent ordered structure and ubiquitinate upon themselves without a substrate and a tag. Ubiquitination experiments using biotinylated ubiquitin showed that the WSTF PHD_EL5 RING finger is poly‐ubiquitinated via residue Lys^63^ of ubiquitin. Substitution of alpha‐helical region might be applicable to various RING fingers with mono‐ubiquitination or poly‐ubiquitination. Copyright © 2011 European Peptide Society and John Wiley & Sons, Ltd.

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