✦ LIBER ✦

Typing and subtyping of herpes simplex isolates by monoclonal fluorescence

✍ Scribed by Dr. S. Sutherland; B. Morgan; A. Mindel; W. L. Chan

Book ID: 102907914
Publisher: John Wiley and Sons
Year: 1986
Tongue: English
Weight: 706 KB
Volume: 18
Category: Article
ISSN: 0146-6615
DOI: 10.1002/jmv.1890180305

No coin nor oath required. For personal study only.

✦ Synopsis

The type specificity of 16 herpes simplex monoclonal antibodies was tested on 50 isolates previously typed by endonuclease restriction. A panel of antibodies was selected and used to type isolates from 50 primary and 50 recurrent genital infections. Sixty-four isolates from 18 patients followed up for six months were subtyped by using a larger panel of monoclonal antibodies and predominant HSVl and HSV2 subtypes were demonstrated. In addition to confirming recurrences, four possible reinfections were identified. Type-specific differences in the location of viral antigens in infected cells were noted and a possible association of this finding with the type-specific differences in rates of recurrence of genital infections discussed.

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