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Types of Collagen Synthesized by Normal Rat Liver Hepatocytes in Primary Culture

✍ Scribed by Scheffer C. G. Tseng; Edward A. Smuckler; Robert Stern

Publisher: John Wiley and Sons
Year: 2007
Tongue: English
Weight: 914 KB
Volume: 3
Category: Article
ISSN: 0270-9139
DOI: 10.1002/hep.1840030613

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✦ Synopsis

Collagen formation is an important function of liver parenchymal cells that may be relevant to the pathogenesis of hepatic fibrosis. The types of collagen synthesized by cultured normal rat liver hepatocytes were examined. Cells isolated from adult rats by enzymatic dispersion of the liver were established in primary monolayer culture. Cells were then incubated with radiolabeled proline for 20 hr in the presence of ascorbate and the lathrogen j3-aminopropionitrile. Collagen secreted into the cell media was assessed separately from that in the cell layer. The greater proportion of newly synthesized collagen was associated with the cell layer. Collagen types were identified by ion exchange chromatography and by polyacrylamide gel electrophoresis. Types I, 111, IV, and V collagen were present in both media and cell layer. Types I11 and V were the predominant types found. Very little Type I collagen was synthesized by these cultured normal hepatocytes. The percentages of Types I, 111, IV, and V collagens, combining media and cell layer, were 6, 38, 19, and 36, respectively.

Collagen is the major component of the interstitial matrix of normal liver (1-3). Disturbances of its metabolism play a role in many forms of chronic liver disease. In particular cirrhosis and in the reaction to toxic injury, 2-to 6-fold increase in the collagen content of the liver occurs. Little is known about the cellular origin of this macromolecule or the types elaborated by specific cell populations in the liver. Additionally, little is known about the regulation of synthesis, or to what degree extracellular collagen can be removed. Development of techniques for the isolation and maintenance in culture of cells from normal rat liver (4-8) make it possible to approach directly the question of the cellular sources of collagen formation.

It has been established in this and other laboratories that normal hepatocytes are able to synthesize collagen

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