Type I collagen regulated dentin matrix protein-1 (Dmp-1) and osteocalcin (OCN) gene expression of rat dental pulp cells

✍ Scribed by Morimichi Mizuno; Tetsuro Miyamoto; Keinoshin Wada; Sanae Watatani; Gui Xia Zhang

• Publisher: John Wiley and Sons
• Year: 2003
• Tongue: English
• Weight: 117 KB
• Volume: 88
• Category: Article
• ISSN: 0730-2312
• DOI: 10.1002/jcb.10466

✦ Synopsis

Abstract

In this study, we investigated the effect of type I collagen on dentin matrix protein‐1 (Dmp‐1) and osteocalcin (OCN) gene expression of dental pulp cells. The mRNA level of Dmp‐1 gene was down‐regulated; however, OCN gene expression was up‐regulated by the culture of dental pulp cells with type I collagen. These findings imply that type I collagen regulates mRNA level of Dmp‐1 and OCN gene that are predominantly expressed in active odontoblasts. The change of gene expression by type I collagen was suppressed by the blocking of collagen–integrin interaction. We could conclude that the effect of type I collagen was mediated via binding of collagen to integrin receptors. J. Cell. Biochem. 88: 1112–1119, 2003. © 2003 Wiley‐Liss, Inc.