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Two theoretical problems concerning the irreversible modification kinetics of enzyme activity

✍ Scribed by Zhi-Xin Wang

Publisher: Elsevier Science
Year: 1990
Tongue: English
Weight: 459 KB
Volume: 142
Category: Article
ISSN: 0022-5193
DOI: 10.1016/s0022-5193(05)80108-0

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✦ Synopsis

Some years ago, a systematic study on the kinetics of irreversible modification of enzyme activity was presented by Tsou (1988, Adv. Enzymol. Relat. Areas molec. Biol. 61, 381-436). This paper presents a discussion of the theoretical aspects of Tsou's method for the determination of the rate constants for the irreversible modification reaction. Range of validity for the kinetic equations of substrate reaction in the presence of the modifier is discussed quantitatively. The expressions of A, B, v* and [P]oo for any enzyme catalyzed reaction mechanism are derived.

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