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Two swinging-bucket ultracentrifugal filters for receptor-binding assays

✍ Scribed by Robert Freundlich; Dermot B. Taylor

Book ID
102626950
Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
449 KB
Volume
105
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

Centrifugal filters for SW 25.1 and 50.1 swinging-bucket ultracentrifuge rotors have been tested up to the maximum speeds allowed, 90,000 and 3OO,OOOg, respectively. The filters are. 1 and 0.5 in. in diameter and accept standard 25mm polycarbonate filter membranes. The filter membranes are both cup-shaped to prevent loss of particulates to the support materials of the filters. The filter for the SW 25.1 rotor can take 0.75 ml and that of the 50.1 head 0.5 ml. The fluid retained after centrifuging consists of the fluid on the filter membrane and in its pores and that retained by the material filtered. The calculated volume of the pores of the 0.2~pm filter was 0.46 ~1. Total liquid retentions of about 0.8 ~1 have been achieved with both filters using a particulate concentration of 0.5 mg/ml.

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