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Two-step procedure for purification and separation of the essential penicillin-binding proteins PBP 1A and 1Bs of Escherichia coli

✍ Scribed by Moritz von Rechenberg; Joachim-Volker Höltje

Book ID
117516475
Publisher
John Wiley and Sons
Year
2000
Tongue
English
Weight
193 KB
Volume
189
Category
Article
ISSN
0378-1097

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✍ Kato, Jun-ichi ;Suzuki, Hideho ;Hirota, Yukinori 📂 Article 📅 1985 🏛 Springer 🌐 English ⚖ 749 KB

A strain of Escherichia coli lacking the entire ponB gene and a strain lacking the proximal part of the ponA gene were constructed by substitution with a drug resistance gene. These strains lost either penicillin-binding protein(PBP)-I b or -la totally and their growth was apparently normal at 30 °