## Abstract Biomolecules are often purified via solvent gradient batch chromatography. Typically suitable smooth linear solvent gradients are applied to obtain the separation between the desired component and hundreds of impurities. The desired product is usually intermediate between weakly and str
Two step capture and purification of IgG2 using multicolumn countercurrent solvent gradient purification (MCSGP)
✍ Scribed by T. Müller-Späth; L. Aumann; G. Ströhlein; H. Kornmann; P. Valax; L. Delegrange; E. Charbaut; G. Baer; A. Lamproye; M. Jöhnck; M. Schulte; M. Morbidelli
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 587 KB
- Volume
- 107
- Category
- Article
- ISSN
- 0006-3592
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## Abstract Multicolumn countercurrent solvent gradient purification (MCSGP) is a continuous chromatographic process developed in recent years (Aumann and Morbidelli, 2007a; Aumann et al., 2007) that is particularly suited for applications in the field of bioseparations. Like batch chromatography,
## Abstract The novel “multicolumn countercurrent solvent gradient purification” (MCSGP) process has been modeled for the purification of a polypeptide mixture characterized by a strong non‐linear competitive adsorption isotherm. As a model system, the purification of an industrial polypeptide mixt