Two simple programs for the analysis of data from enzyme-linked immunosorbent (ELISA) assays on a programmable desk-top calculator
✍ Scribed by D.G. Ritchie; J.M. Nickerson; G.M. Fuller
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 840 KB
- Volume
- 110
- Category
- Article
- ISSN
- 0003-2697
No coin nor oath required. For personal study only.
✦ Synopsis
We have designed two programs for use with an inexpensive programmable calculator which rapidly and accurately convert raw data generated from enzyme-linked immunosorbent assays directly into antigen concentration. The first program computes and compares effective doses (ED,,)'s between a standard and each unknown sample assayed. The EDso from the unknown sample is then multiplied by a concentration factor which yields the unknown concentration. The second program linearizes the sigmoidal enzyme-linked immunosorbent assay titration curve using a logit-log transformation of the data in order to compute unknown concentration values. Both programs employ stringent limit conditions to decrease "nonsense" calculations. Data are then processed by a least-squares best-fit linear regression analysis.