Specific fluorescent labeling of two fun
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Olga N. Ozoline; Katsuhiko Murakami; Tomofumi Negishi; Nobuyuki Fujita; Akira Is
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Article
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1998
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John Wiley and Sons
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English
⚖ 231 KB
A monomercury derivative of fluoresceine acetate (FMMA) was previously suggested as a specific reagent reacting with only one of four cysteine (Cys) residues in the ␣ subunit of Escherichia coli RNA polymerase. Here, we analyzed the reactivity against FMMA of both isolated ␣ subunit and ␣ subunit as