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Two populations of β-spectrin in rat skeletal muscle

✍ Scribed by Porter, George A. ;Scher, Malka G. ;Resneck, Wendy G. ;Porter, Neil C. ;Fowler, Velia M. ;Bloch, Robert J.

Publisher
John Wiley and Sons
Year
1997
Tongue
English
Weight
367 KB
Volume
37
Category
Article
ISSN
0886-1544

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✦ Synopsis

We use immunoblotting, immunoprecipitation, and centrifugation in sucrose density gradients to show that the product of the erythrocyte beta-spectrin gene in rat skeletal muscle (muscle beta-spectrin) is present in two states, one associated with fodrin, and another that is not associated with any identifiable spectrin or fodrin subunit. Immunofluorescence studies indicate that a significant amount of beta-spectrin without alpha-fodrin is present in the myoplasm of some muscle fibers, and, more strikingly, at distinct regions of the sarcolemma. These results suggest that alpha-fodrin and muscle beta-spectrin associate in muscle in situ, but that some muscle beta-spectrin without a paired alpha-subunit forms distinct domains at the sarcolemma.

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