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Two-Photon Thermal Bleaching of Single Fluorescent Molecules

✍ Scribed by Chirico, Giuseppe; Cannone, Fabio; Baldini, Giancarlo; Diaspro, Alberto

Book ID
118515623
Publisher
Biophysical Society
Year
2003
Tongue
English
Weight
225 KB
Volume
84
Category
Article
ISSN
0006-3495

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πŸ“œ SIMILAR VOLUMES

Single molecule studies by means of the
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## Abstract We have characterized a commercial confocal scanning head for the detection of single molecule fluorescence by two‐photon excitation. We have verified that the distribution of the fluorescence emitted by dyes and labeled proteins on glass substrates is discrete with quanta proportional

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## Abstract __We report the two‐photon excitation and emission of a recently developed green fluorescent protein (GFP) mutant, E__^__2__^__GFP. Two main excitation bands are found at 780 and 870 nm. Blinking and irreversible and reversible bleaching were observed. Fluorescence blinking occurs in th