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Two-Photon Fluorescence Coincidence Analysis: Rapid Measurements of Enzyme Kinetics

✍ Scribed by Heinze, Katrin G.; Rarbach, Markus; Jahnz, Michael; Schwille, Petra


Book ID
118514599
Publisher
Biophysical Society
Year
2002
Tongue
English
Weight
345 KB
Volume
83
Category
Article
ISSN
0006-3495

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## Background: The Cellscan mark-S (CS-S) scanning cytometer was used for tracing enzymatic reactions in the same individual cells under various physiological conditions over periods of minutes. On-line reagent addition and changes in the experimental conditions (buffers, ions, substrates and inhib