Two-photon excited hemoglobin fluorescence

✍ Scribed by Zheng, Wei; Li, Dong; Zeng, Yan; Luo, Yi; Qu, Jianan Y.

• Book ID: 115366324
• Publisher: The Optical Society
• Year: 2010
• Tongue: English
• Weight: 879 KB
• Volume: 2
• Category: Article
• ISSN: 2156-7085
• DOI: 10.1364/BOE.2.000071

✦ Synopsis

We discovered that hemoglobin emits high energy Soret fluorescence when two-photon excited by the visible femtosecond light sources. The unique spectral and temporal characteristics of hemoglobin fluorescence were measured by using a time-resolved spectroscopic detection system. The high energy Soret fluorescence of hemoglobin shows the spectral peak at 438 nm with extremely short lifetime. This discovery enables two-photon excitation fluorescence microscopy to become a potentially powerful tool for in vivo label-free imaging of blood cells and vessels.